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Identification of Optimal Concentrations and Incubation Times for the Study of In Vitro Effects of Pb in Ram Spermatozoa

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dc.contributor.author Castellanos, Pilar
dc.contributor.author Maroto Morales, Alejandro
dc.contributor.author García Alvarez, Olga
dc.contributor.author Garde López-Brea, José Julián
dc.contributor.author Mateo, Rafael
dc.date.accessioned 2015-10-19T12:38:33Z
dc.date.available 2015-10-19T12:38:33Z
dc.date.issued 2013
dc.identifier.citation Bulletin of Environmental Contamination and Toxicology (2013) 91:197–201
dc.identifier.issn 0007-4861
dc.identifier.uri http://hdl.handle.net/10578/7293
dc.description.abstract In vitro effects of lead (Pb) on ram (Ovis aries) spermatozoa were studied to establish a threshold level that affects sperm function. Spermatozoa were incubated between 15 and 180 min with Pb concentrations ranging from 0 to 5,000 ng/mL. Sperm motility, acrosome integrity, membrane functionality and sperm viability were all negatively affected by Pb and incubation time. Acrosome integrity was linearly affected by Pb levels at an incubation time of 30 min, and 50 ng/mL was the lowest Pb level producing such effect. These experimental conditions can be appropriate for in vitro studies of the mechanisms of action of Pb on spermatozoa. es_ES
dc.format application/pdf es_ES
dc.language.iso en es_ES
dc.rights info:eu-repo/semantics/openAccess es_ES
dc.subject Lead es_ES
dc.subject Spermatozoa es_ES
dc.subject Semen quality es_ES
dc.subject Acrosome integrity es_ES
dc.title Identification of Optimal Concentrations and Incubation Times for the Study of In Vitro Effects of Pb in Ram Spermatozoa es_ES
dc.type info:eu-repo/semantics/article es_ES
dcterms.identifier DOI 10.1007/s00128-013-1032-5


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