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Fertility of cryopreserved ovine semen is determined by sperm velocity.

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dc.contributor.author Olmo Medina, Enrique del
dc.contributor.author Bisbal, A.
dc.contributor.author Maroto Morales, Alejandro
dc.contributor.author García Álvarez, Olga
dc.contributor.author Ramón, Manuel
dc.contributor.author Jiménez Rabadán, Pilar
dc.contributor.author Martínez-Pastor, F.
dc.contributor.author Soler, Ana Josefa
dc.contributor.author Garde López-Brea, José Julián
dc.contributor.author Fernández Santos, María del Rocío
dc.date.accessioned 2015-10-30T12:54:59Z
dc.date.available 2015-10-30T12:54:59Z
dc.date.issued 2013-04
dc.identifier.citation Animal Reproduction Science (2013), vol. 138, n. 1-2, pp. 102-109
dc.identifier.issn 0378-4320
dc.identifier.uri http://hdl.handle.net/10578/7437
dc.description.abstract The present study aims to examine the predictive value of some sperm parameters on male fertility. Semen samples from six Manchega rams were collected and cryopreserved. Sperm quality was assessed after thawing and after 2 h of incubation, either in the freezing extender (37#9702;C) or after dilution in synthetic Oviductal Fluid (SOF) (38 #9702;C, 5% CO2), attempting to mimic the physiological conditions of the female reproductive tract. The following sperm parameters were evaluated: motility and kinetic parameters by computer-assisted semen analyzer (CASA), and sperm viability (propidium iodide), mitochondrial membrane potential (JC-1), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), and intracellular calcium (fluo-3) by flow cytometry. Results showed no significant differences between incubation media neither after thawing nor after incubation. There were no significant correlations between fertility and sperm parameters assessed by flow cytometry. However, after incubation in the freezing extender, sperm samples from males with por fertility yielded less linearity and velocity (P < 0.05) as indicated by motility parameters analyzed by CASA. These results indicate that kinematic sperm motility parameters evaluation by CASA might be useful to identify samples with poor fertility. es_ES
dc.format application/pdf es_ES
dc.language.iso en es_ES
dc.publisher Elsevier es_ES
dc.rights info:eu-repo/semantics/openAccess es_ES
dc.subject Ganado ovino es_ES
dc.subject Espermatozoides es_ES
dc.subject Crioconservación es_ES
dc.title Fertility of cryopreserved ovine semen is determined by sperm velocity. es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.identifier.DOI 10.1016/j.anireprosci.2013.02.007


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